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Wright's stain

CAS No.: 68988-92-1
Formula: C36H27Br4N3O5S+2
Molecular Weight: 933.29768
Suppliers: All(0) China Suppliers(2) Products(6)
  • Description
  • Basic Info
  • Safety Info
  • MSDS
  • Price
  • Related Product
  • Supplier Reference
What is Wright's stain

**Introduction to Wright’s Stain** Wright’s stain is a widely used Romanowsky-type stain in hematology and cytology, renowned for its ability to differentiate blood cells and detect abnormalities in peripheral blood smears. Composed of methylene blue and eosin dyes, it produces a distinctive color spectrum—nuclei stain purple, cytoplasm appears blue to pink, and granules in specific cells (e.g., neutrophils, eosinophils) stand out vividly. This stain is essential for diagnosing conditions like anemia, infections, and leukemia. Its consistent performance, ease of use, and compatibility with automated staining systems make it a staple in clinical and research laboratories. Wright’s stain remains a cornerstone tool for accurate morphological analysis in hematologic diagnostics.

Preparation Process: To prepare Wright's stain, dissolve 0.3 g of Wright’s stain powder in 100 mL of absolute methanol. Stir thoroughly until fully dissolved, then filter the solution to remove undissolved particles. Store the filtered solution in a dark, airtight glass bottle to prevent oxidation and evaporation. For use, dilute the stock solution 1:1 with pH 6.8 phosphate buffer before staining. Alternatively, prepare a working solution by mixing 0.25 g Wright’s stain powder with 60 mL methanol and 10 mL glycerol, then add 30 mL pH 6.8 buffer. Filter and store in a brown bottle. Ensure consistent staining by maintaining proper pH and avoiding light exposure.

Usage Scenarios: Wright's stain is a commonly used histological and hematological dye that combines eosin and methylene blue to differentiate blood cell types in peripheral blood smears and bone marrow samples. It stains red blood cells pink to red, nuclei blue to purple, and cytoplasmic granules in white blood cells varying shades of blue, pink, or purple, aiding in the identification of cell types and abnormalities. The stain is widely used in clinical laboratories for diagnosing conditions like anemia, leukemia, and infections. It also helps in malaria diagnosis by highlighting parasitic inclusions within red blood cells. Wright's stain provides clear cellular detail, making it essential for microscopic examination of blood and bone marrow specimens.

Wright's stain Basic Info
Chemical Name Wright's stain
Synonyms wright’s;Wright's sta;WRIGHT STAIN;WrightpigMent;WRIGHTS STAIN;WRIGHT'S STAIN;Wrigh's stain;Wright's stain;Wright's Staine;BUFFER GIORDANO
CAS No. 68988-92-1
Molecular Formula C36H27Br4N3O5S+2
Molecular Weight 933.29768
PSA 138.51000
LogP 11.55610
Safely Info
RTECS -
Hazard Class -
Safety Statements 26-39-61-24/25-7-2-16-37/39
HS Code 32041900
WGK Germany 3
Packing Group II
RIDADR -
Risk Statements 22-41-52/53-23/25-11-36/37/38
Hazard Codes Xn,T,F,Xi
Caution Statement P280-P301+P312+P330-P305+P351+P338-P337+P313
Hazard Declaration H302-H319
Symbol GHS07
Signal Word Warning
Wright's stain Price
Here is a rough price range for Wright's stain in the 18 countries listed. Note that prices can vary based on suppliers, quantity, and local market conditions:

1. **United States**: $10 - $50
2. **China**: $5 - $30
3. **Russia**: $10 - $40
4. **Germany**: €10 - €50
5. **India**: ₹500 - ₹2000
6. **Japan**: ¥1000 - ¥5000
7. **Brazil**: R$50 - R$200
8. **South Korea**: ₩10,000 - ₩50,000
9. **Philippines**: ₱500 - ₱2000
10. **United Kingdom**: £10 - £50
11. **France**: €10 - €50
12. **Mexico**: MXN$200 - MXN$800
13. **Canada**: CAD$15 - CAD$60
14. **South Africa**: ZAR200 - ZAR800
15. **Egypt**: EGP200 - EGP800
16. **Turkey**: ₺100 - ₺400
17. **Thailand**: ฿300 - ฿1200
18. **Indonesia**: IDR100,000 - IDR400,000

If no specific price range is available for a country, it is marked as "No results." Let me know if you need further clarification!
MSDS
MSDS

SECTION 1: Identification of the substance/mixture and of the company/undertaking
  Product identifiers
    Product name        : Wright stain    
    REACH No.        : A registration number is not available for this substance as the substance    
    or its uses are exempted from registration, the annual tonnage does not
    require a registration or the registration is envisaged for a later
    registration deadline.
    CAS-No.        : 68988-92-1    
  Relevant identified uses of the substance or mixture and uses advised against
    Identified uses        : Laboratory chemicals, Manufacture of substances    
  
  

SECTION 2: Hazards identification
  Classification of the substance or mixture
  Classification according to Regulation (EC) No 1272/2008
    Acute toxicity, Oral (Category 4), H302
    Serious eye damage (Category 1), H318
    For the full text of the H-Statements mentioned in this Section, see Section 16.
  Classification according to EU Directives 67/548/EEC or 1999/45/EC
    Xn Harmful        R22    
    Xi Irritant        R41    
    R52/53
    For the full text of the R-phrases mentioned in this Section, see Section 16.
  Label elements
  Labelling according Regulation (EC) No 1272/2008
    Pictogram
    Signal word        Danger    
    Hazard statement(s)
    H302        Harmful if swallowed.    
    H318        Causes serious eye damage.    
    Precautionary statement(s)
    P280        Wear protective gloves/ eye protection/ face protection.    
    P305 + P351 + P338        IF IN EYES: Rinse cautiously with water for several minutes. Remove    
    contact lenses, if present and easy to do. Continue rinsing.
    Supplemental Hazard        none    
    Statements
  According to European Directive 67/548/EEC as amended.
    Hazard symbol(s)        Xn Harmful    
    R-phrase(s)
    R22        Harmful if swallowed.    
    R41        Risk of serious damage to eyes.    
    R52/53        Harmful to aquatic organisms, may cause long-term adverse effects in    
    the aquatic environment.
    S-phrase(s)
    S26        In case of contact with eyes, rinse immediately with plenty of water and    
    seek medical advice.
    S39        Wear eye/face protection.    
    S61        Avoid release to the environment. Refer to special instructions/ Safety    
    data sheets.
  Other hazards - none

SECTION 3: Composition/information on ingredients
  Mixtures
    Synonyms        : Eosin Methylene blue according to Wright    
  Hazardous ingredients according to Regulation (EC) No 1272/2008
    Component        Classification        Concentration    
  Methylthioninium chloride
    CAS-No.        61-73-4        Acute Tox. 4; Eye Dam. 1;        50 - 100 %    
    EC-No.        200-515-2        H302, H318    
  Disodium 2-(2,4,5,7-tetrabromo-6-oxido-3-oxoxanthen-9-yl)benzoate
    CAS-No.        17372-87-1        Eye Irrit. 2; H319        25 - 50 %    
    EC-No.        241-409-6    
  Hazardous ingredients according to Directive 1999/45/EC
    Component        Classification        Concentration    
  Methylthioninium chloride
    CAS-No.        61-73-4        Xn, R22 - R41 - R52/53        50 - 100 %    
    EC-No.        200-515-2    
  Disodium 2-(2,4,5,7-tetrabromo-6-oxido-3-oxoxanthen-9-yl)benzoate
    CAS-No.        17372-87-1        Xi, R36        25 - 50 %    
    EC-No.        241-409-6    
    For the full text of the H-Statements and R-Phrases mentioned in this Section, see Section 16

SECTION 4: First aid measures
  Description of first aid measures
  General advice
    Consult a physician. Show this safety data sheet to the doctor in attendance.
  If inhaled
    If breathed in, move person into fresh air. If not breathing, give artificial respiration. Consult a physician.
  In case of skin contact
    Wash off with soap and plenty of water. Consult a physician.
  In case of eye contact
    Rinse thoroughly with plenty of water for at least 15 minutes and consult a physician.
  If swallowed
    Never give anything by mouth to an unconscious person. Rinse mouth with water. Consult a physician.
  Most important symptoms and effects, both acute and delayed
    The most important known symptoms and effects are described in the labelling (see section 2.2) and/or in
    section 11
  Indication of any immediate medical attention and special treatment needed
    no data available

SECTION 5: Firefighting measures
  Extinguishing media
  Suitable extinguishing media
    Use water spray, alcohol-resistant foam, dry chemical or carbon dioxide.
  Special hazards arising from the substance or mixture
    Carbon oxides, nitrogen oxides (NOx), Sulphur oxides, Hydrogen chloride gas, Hydrogen bromide gas,
    Sodium oxides
  Advice for firefighters
    Wear self contained breathing apparatus for fire fighting if necessary.
  Further information
    no data available

SECTION 6: Accidental release measures
  Personal precautions, protective equipment and emergency procedures
    Use personal protective equipment. Avoid dust formation. Avoid breathing vapours, mist or gas. Ensure
    adequate ventilation. Evacuate personnel to safe areas. Avoid breathing dust.
    For personal protection see section 8.
  Environmental precautions
    Prevent further leakage or spillage if safe to do so. Do not let product enter drains. Discharge into the
    environment must be avoided.
  Methods and materials for containment and cleaning up
    Pick up and arrange disposal without creating dust. Sweep up and shovel. Keep in suitable, closed
    containers for disposal.
  Reference to other sections
    For disposal see section 13.

SECTION 7: Handling and storage
  Precautions for safe handling
    Avoid contact with skin and eyes. Avoid formation of dust and aerosols.
    Provide appropriate exhaust ventilation at places where dust is formed.Normal measures for preventive fire
    protection.
    For precautions see section 2.2.
  Conditions for safe storage, including any incompatibilities
    Store in cool place. Keep container tightly closed in a dry and well-ventilated place.
  Specific end use(s)
    A part from the uses mentioned in section 1.2 no other specific uses are stipulated

SECTION 8: Exposure controls/personal protection
  Control parameters
  Components with workplace control parameters
  Exposure controls
  Appropriate engineering controls
    Handle in accordance with good industrial hygiene and safety practice. Wash hands before breaks and
    at the end of workday.
  Personal protective equipment
  Eye/face protection
    Face shield and safety glasses Use equipment for eye protection tested and approved under
    appropriate government standards such as NIOSH (US) or EN 166(EU).
  Skin protection
    Handle with gloves. Gloves must be inspected prior to use. Use proper glove removal technique
    (without touching glove's outer surface) to avoid skin contact with this product. Dispose of
    contaminated gloves after use in accordance with applicable laws and good laboratory practices.
    Wash and dry hands.
    The selected protective gloves have to satisfy the specifications of EU Directive 89/686/EEC and
    the standard EN 374 derived from it.
  Body Protection
    Complete suit protecting against chemicals, The type of protective equipment must be selected
    according to the concentration and amount of the dangerous substance at the specific workplace.
  Respiratory protection
    Where risk assessment shows air-purifying respirators are appropriate use a full-face particle
    respirator type N100 (US) or type P3 (EN 143) respirator cartridges as a backup to engineering
    controls. If the respirator is the sole means of protection, use a full-face supplied air respirator. Use
    respirators and components tested and approved under appropriate government standards such
    as NIOSH (US) or CEN (EU).
  Control of environmental exposure
    Prevent further leakage or spillage if safe to do so. Do not let product enter drains. Discharge into
    the environment must be avoided.

SECTION 9: Physical and chemical properties
  Information on basic physical and chemical properties
    a) Appearance        Form: powder    
    Colour: dark green
    b) Odour        no data available    
    c) Odour Threshold        no data available    
    d) pH        no data available    
    e) Melting point/freezing        no data available    
    point
    f) Initial boiling point and no data available
    boiling range
    g) Flash point        no data available    
    h) Evapouration rate        no data available    
    i) Flammability (solid, gas) no data available
    j) Upper/lower        no data available    
    flammability or
    explosive limits
    k) Vapour pressure        no data available    
    l) Vapour density        no data available    
    m) Relative density        no data available    
    n) Water solubility        no data available    
    o) Partition coefficient: n- no data available
    octanol/water
    p) Auto-ignition        no data available    
    temperature
    q) Decomposition        no data available    
    temperature
    r) Viscosity        no data available    
    s) Explosive properties        no data available    
    t) Oxidizing properties        no data available    
  Other safety information
    no data available

SECTION 10: Stability and reactivity
  Reactivity
    no data available
  Chemical stability
    Stable under recommended storage conditions.
  Possibility of hazardous reactions
    no data available
  Conditions to avoid
    no data available
  Incompatible materials
    Bases, Strong oxidizing agents, Reducing agents
  Hazardous decomposition products
    Other decomposition products - no data available
    In the event of fire: see section 5

SECTION 11: Toxicological information
  Information on toxicological effects
  Acute toxicity
    no data available
  Skin corrosion/irritation
    no data available
  Serious eye damage/eye irritation
    no data available
  Respiratory or skin sensitisation
    no data available
  Germ cell mutagenicity
    no data available
  Carcinogenicity
    IARC:        No component of this product present at levels greater than or equal to 0.1% is identified as    
    probable, possible or confirmed human carcinogen by IARC.
  Reproductive toxicity
    no data available
  Specific target organ toxicity - single exposure
    no data available
  Specific target organ toxicity - repeated exposure
    no data available
  Aspiration hazard
    no data available
  Additional Information
    RTECS: Not available
    Abdominal pain, Nausea, Dizziness, Headache, To the best of our knowledge, the chemical, physical, and
    toxicological properties have not been thoroughly investigated.

SECTION 12: Ecological information
  Toxicity
    no data available
  Persistence and degradability
    no data available
  Bioaccumulative potential
    no data available
  Mobility in soil
    no data available
  Results of PBT and vPvB assessment
    PBT/vPvB assessment not available as chemical safety assessment not required/not conducted
  Other adverse effects
    Harmful to aquatic life.

SECTION 13: Disposal considerations
  Waste treatment methods
  Product
    Offer surplus and non-recyclable solutions to a licensed disposal company. Contact a licensed
    professional waste disposal service to dispose of this material. Dissolve or mix the material with a
    combustible solvent and burn in a chemical incinerator equipped with an afterburner and scrubber.
  Contaminated packaging
    Dispose of as unused product.

SECTION 14: Transport information
  UN number
    ADR/RID: -        IMDG: -        IATA: -    
  UN proper shipping name
    ADR/RID: Not dangerous goods
    IMDG: Not dangerous goods
    IATA:        Not dangerous goods    
  Transport hazard class(es)
    ADR/RID: -        IMDG: -        IATA: -    
  Packaging group
    ADR/RID: -        IMDG: -        IATA: -    
  Environmental hazards
    ADR/RID: no        IMDG Marine pollutant: no        IATA: no    
  Special precautions for user
    no data available



SECTION 15 - REGULATORY INFORMATION
N/A


SECTION 16 - ADDITIONAL INFORMATION
N/A
SDS 1.0
Wright's stain Suppliers
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China Liaoning Shenyang
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China Shanghai Districts
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