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        Giemsa stain

        CAS No.: 51811-82-6
        Formula: C14Cl1H14N3S1
        Molecular Weight: 291.8
        Suppliers: All(0) China Suppliers(3) Products(10)
        • Description
        • Basic Info
        • Safety Info
        • MSDS
        • Price
        • Related Product
        • Supplier Reference
        What is Giemsa stain

        **Introduction to Giemsa Stain** Giemsa stain is a widely used Romanowsky-type dye in microbiology, hematology, and cytogenetics. Composed of methylene blue, eosin, and azure B, it is renowned for its ability to differentially stain cellular components, particularly blood films and tissue samples. Giemsa stain is essential for identifying malaria parasites, differentiating blood cell types, and analyzing chromosomal abnormalities (G-banding). Its high affinity for DNA and RNA allows clear visualization of nuclear and cytoplasmic structures under a microscope. With consistent, reliable results, Giemsa stain remains a cornerstone in diagnostic laboratories, research, and clinical settings. Proper staining techniques ensure optimal contrast, making it indispensable for pathological and hematological studies.

        Preparation Process: To prepare Giemsa stain, dissolve 0.5 g of Giemsa powder in 33 mL of glycerol at 56°C for 2 hours. Add 33 mL of methanol (absolute) and mix thoroughly. Filter the solution to remove undissolved particles. For working solution, dilute 1 part stock with 9 parts phosphate buffer (pH 6.8) or distilled water. Alternatively, dissolve 1 g Giemsa powder in 66 mL glycerol, heat to 50–60°C for 30 minutes, then add 66 mL methanol, mix, and filter. Store stock solution in a dark bottle. Use fresh working solution for staining blood smears (5–10 minutes).

        Usage Scenarios: Giemsa stain is a widely used histological and cytological dye primarily employed to visualize cellular and microbial components under a microscope. It is commonly applied in hematology to differentiate blood cells, identify malarial parasites, and diagnose blood disorders like leukemia. In microbiology, it aids in detecting bacteria, spirochetes, and rickettsiae. Giemsa stain is also utilized in cytogenetics for chromosome banding (G-banding) to analyze chromosomal abnormalities. Additionally, it helps in identifying fungal and protozoan infections. The stain works by binding to nucleic acids and proteins, producing distinct color contrasts—blue for nuclei and pink for cytoplasm—enhancing microscopic examination of cellular structures.

        Giemsa stain Basic Info
        Chemical Name Giemsa stain
        Synonyms GIEMSA L;GIEMSA R;GieMsa stai;GIEMSA STAIN;Giemse Stain;im's pigment;im's pigment;GIEMSA BUFFER;GieMsa's stai;Giema's stain
        CAS No. 51811-82-6
        Molecular Formula C14Cl1H14N3S1
        Molecular Weight 291.8
        PSA 70.39000
        LogP 0.96400
        Safely Info
        RTECS -
        Hazard Class -
        Safety Statements 7-16-36/37-45-39-26-53-25-61
        HS Code 32129000
        WGK Germany 3
        Packing Group -
        RIDADR UN 1230 3/PG 2
        Risk Statements 41-39/23/24/25-23/24/25-11-63-62-46-36/38-21-52/53-22
        Hazard Codes Xi,T,F,Xn
        Caution Statement P210-P260-P280-P310-P301+P310-P311
        Hazard Declaration H225-H301+H311+H331-H370
        Symbol GHS02,GHS06,GHS08
        Signal Word Danger
        Giemsa stain Price
        Here is a rough price range for Giemsa stain in the listed countries. Note that prices can vary based on suppliers, quantities, and local market conditions:

        - **United States**: $20–$50 per 100 mL
        - **China**: $10–$30 per 100 mL
        - **Russia**: $15–$40 per 100 mL
        - **Germany**: $25–$60 per 100 mL
        - **India**: $5–$20 per 100 mL
        - **Japan**: $30–$70 per 100 mL
        - **Brazil**: $20–$50 per 100 mL
        - **South Korea**: $25–$55 per 100 mL
        - **Philippines**: $15–$40 per 100 mL
        - **United Kingdom**: $30–$65 per 100 mL
        - **France**: $25–$60 per 100 mL
        - **Mexico**: $20–$45 per 100 mL
        - **Canada**: $25–$60 per 100 mL
        - **South Africa**: $15–$40 per 100 mL
        - **Egypt**: $10–$30 per 100 mL
        - **Turkey**: $15–$40 per 100 mL
        - **Thailand**: $10–$35 per 100 mL
        - **Indonesia**: $10–$30 per 100 mL

        If no specific price data is available for a country, it is marked as "No results." Prices are approximate and may vary.
        MSDS
        MSDS
        Name: Geimsa Stain Material Safety Data Sheet
        Synonym: None known
        CAS: 51811-82-6
        Section 1 - Chemical Product   MSDS Name:Geimsa Stain Material Safety Data Sheet
        Synonym:None known
        Section 2 - COMPOSITION, INFORMATION ON INGREDIENTS
        CAS# Chemical Name content EINECS#
        51811-82-6 Giemsa Stain 100.0 257-438-2
        Hazard Symbols: None Listed.
        Risk Phrases: None Listed.
        Section 3 - HAZARDS IDENTIFICATION
          EMERGENCY OVERVIEW
        The toxicological properties of this material have not been fully investigated.
        Potential Health Effects
        Eye:
        May cause eye irritation. This product contains a cationic dye.
        Similar dyes have caused permanent injury to the cornea and conjunctiva in documented exposure cases with human or rabbit eyes.
        Skin:
        May cause skin irritation.
        Ingestion:
        May cause irritation of the digestive tract. Low hazard for usual industrial handling. The toxicological properties of this substance have not been fully investigated.
        Inhalation:
        May cause respiratory tract irritation. Low hazard for usual industrial handling. The toxicological properties of this substance have not been fully investigated.
        Chronic:
        No information found.
        Section 4 - FIRST AID MEASURES
          Eyes: Flush eyes with plenty of water for at least 15 minutes, occasionally lifting the upper and lower eyelids. Get medical aid.
        Skin:
        Flush skin with plenty of water for at least 15 minutes while removing contaminated clothing and shoes. Get medical aid if irritation develops or persists. Wash clothing before reuse.
        Ingestion:
        Never give anything by mouth to an unconscious person. Get medical aid. Do NOT induce vomiting. If conscious and alert, rinse mouth and drink 2-4 cupfuls of milk or water.
        Inhalation:
        Remove from exposure and move to fresh air immediately. If not breathing, give artificial respiration. If breathing is difficult, give oxygen. Get medical aid if cough or other symptoms appear.
        Notes to Physician:
        Section 5 - FIRE FIGHTING MEASURES
          General Information:
        As in any fire, wear a self-contained breathing apparatus in pressure-demand, MSHA/NIOSH (approved or equivalent), and full protective gear. During a fire, irritating and highly toxic gases may be generated by thermal decomposition or combustion.
        Extinguishing Media:
        Use agent most appropriate to extinguish fire. Use water spray, dry chemical, carbon dioxide, or appropriate foam.
        Section 6 - ACCIDENTAL RELEASE MEASURES
          General Information: Use proper personal protective equipment as indicated in Section 8.
        Spills/Leaks:
        Vacuum or sweep up material and place into a suitable disposal container. Clean up spills immediately, observing precautions in the Protective Equipment section. Avoid generating dusty conditions.
        Provide ventilation.
        Section 7 - HANDLING and STORAGE
          Handling:
        Wash thoroughly after handling. Use with adequate ventilation.
        Minimize dust generation and accumulation. Avoid contact with eyes, skin, and clothing. Keep container tightly closed. Avoid ingestion and inhalation.
        Storage:
        Store in a tightly closed container. Store in a cool, dry, well-ventilated area away from incompatible substances.
        Section 8 - EXPOSURE CONTROLS, PERSONAL PROTECTION
          Engineering Controls:
        Facilities storing or utilizing this material should be equipped with an eyewash facility and a safety shower. Use adequate ventilation to keep airborne concentrations low.
        Exposure Limits CAS# 51811-82-6: Personal Protective Equipment Eyes: Wear appropriate protective eyeglasses or chemical safety goggles as described by OSHA's eye and face protection regulations in 29 CFR 1910.133 or European Standard EN166.
        Skin:
        Wear appropriate protective gloves to prevent skin exposure.
        Clothing:
        Wear appropriate protective clothing to prevent skin exposure.
        Respirators:
        A respiratory protection program that meets OSHA's 29 CFR 1910.134 and ANSI Z88.2 requirements or European Standard EN 149 must be followed whenever workplace conditions warrant respirator use.
        Section 9 - PHYSICAL AND CHEMICAL PROPERTIES
         
        Physical State: Solid
        Color: Dark green fine crystalline powder
        Odor: none reported
        pH: Not available.
        Vapor Pressure: Not available.
        Viscosity: Not available.
        Boiling Point: Not available.
        Freezing/Melting Point: 300 deg C
        Autoignition Temperature: Not applicable.
        Flash Point: Not applicable.
        Explosion Limits, lower: Not available.
        Explosion Limits, upper: Not available.
        Decomposition Temperature:
        Solubility in water: Soluble.
        Specific Gravity/Density:
        Molecular Formula:
        Molecular Weight:
        Section 10 - STABILITY AND REACTIVITY
          Chemical Stability:
        Stable under normal temperatures and pressures.
        Conditions to Avoid:
        Incompatible materials, dust generation, excess heat.
        Incompatibilities with Other Materials:
        Oxidizing agents, nitric acid, sulfuric acid.
        Hazardous Decomposition Products:
        Carbon monoxide, irritating and toxic fumes and gases, carbon dioxide.
        Hazardous Polymerization: Has not been reported.
        Section 11 - TOXICOLOGICAL INFORMATION
          RTECS#:
        CAS# 51811-82-6 unlisted.
        LD50/LC50:
        Not available.
        Carcinogenicity:
        Giemsa Stain - Not listed by ACGIH, IARC, or NTP.
        Section 12 - ECOLOGICAL INFORMATION
         
        Section 13 - DISPOSAL CONSIDERATIONS
          Dispose of in a manner consistent with federal, state, and local regulations.
        Section 14 - TRANSPORT INFORMATION
         
        IATA
        No information available.
        IMO
        No information available.
        RID/ADR
        No information available.
        Section 15 - REGULATORY INFORMATION
         
        European/International Regulations
        European Labeling in Accordance with EC Directives
        Hazard Symbols: Not available.
        Risk Phrases:
        Safety Phrases:
        S 28A After contact with skin, wash immediately with
        plenty of water.
        S 37 Wear suitable gloves.
        S 45 In case of accident or if you feel unwell, seek
        medical advice immediately (show the label where
        possible).
        WGK (Water Danger/Protection)
        CAS# 51811-82-6: No information available.
        Canada
        CAS# 51811-82-6 is listed on Canada's DSL List.
        CAS# 51811-82-6 is not listed on Canada's Ingredient Disclosure List.
        US FEDERAL
        TSCA
        CAS# 51811-82-6 is listed on the TSCA inventory.

        SECTION 16 - ADDITIONAL INFORMATION
        N/A
        SDS 1.0
        Giemsa stain Suppliers
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        A&K Petro-Chem Ind. Ltd.
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