**Introduction to Inulin, 14C-Carboxylated** Inulin, 14C-carboxylated, is a radiolabeled polysaccharide derived from natural inulin, a fructan commonly found in plants like chicory root. This specialized compound is carboxylated and tagged with carbon-14 (14C) at specific positions, making it an invaluable tool for metabolic and pharmacokinetic studies. Researchers use 14C-labeled inulin to track absorption, distribution, and excretion in biological systems, particularly in renal function tests, gut microbiota studies, and carbohydrate metabolism research. Its high purity and precise labeling ensure reliable data in tracer experiments. Ideal for both *in vitro* and *in vivo* applications, this product supports advancements in nutrition, pharmacology, and biomedical sciences. (Word count: ~100)
Preparation Process: To prepare **Inulin, 14C-Carboxylated**, follow these steps: 1. **Substrate Selection**: Use high-purity inulin as the starting material. 2. **Radiolabeling**: Introduce 14C at the carboxyl group via carboxylation using 14CO2 under controlled conditions (e.g., enzymatic or chemical carboxylation with carboxylase enzymes or strong bases like KOH). 3. **Purification**: Isolate the labeled product using dialysis, gel filtration chromatography, or precipitation to remove unreacted 14CO2 and byproducts. 4. **Characterization**: Verify purity and labeling efficiency via HPLC, scintillation counting, or mass spectrometry. 5. **Storage**: Lyophilize and store at –20°C to preserve stability. (Word count: 100)
Usage Scenarios: Inulin, 14C-carboxylated, is a radiolabeled compound primarily used in metabolic and pharmacokinetic studies to trace carbohydrate metabolism and assess digestive function. The 14C labeling allows precise tracking of inulin's distribution, absorption, and excretion in biological systems. It serves as a diagnostic tool to measure glomerular filtration rate (GFR) in renal function tests due to its inert nature and exclusive renal clearance. Researchers also employ it to study gut microbiota fermentation, as inulin is a prebiotic fiber metabolized by colonic bacteria. Additionally, it aids in investigating intestinal permeability and carbohydrate metabolism pathways in both preclinical and clinical settings.