ATP K+ salt

What is ATP K+ salt

ATP K+ salt, or Adenosine Triphosphate Potassium Salt, is a high-purity biochemical reagent widely used in molecular biology, biochemistry, and cellular research. As the primary energy currency of cells, ATP plays a critical role in energy transfer and metabolic processes. The potassium salt form enhances solubility and stability, making it ideal for experimental applications such as enzyme kinetics, cell signaling studies, and energy metabolism research. ATP K+ salt is essential for in vitro assays, including luciferase-based bioluminescence assays, and is a key component in studies involving ATP-dependent enzymes and cellular energy dynamics. Its reliable performance and consistent quality make it a trusted choice for researchers worldwide.

Preparation Process: To prepare ATP K⁺ salt, dissolve adenosine triphosphate (ATP) disodium salt in distilled water. Adjust the pH to 7.0–7.5 using a potassium hydroxide (KOH) solution. Pass the solution through a cation-exchange resin (e.g., Dowex 50W-X8, K⁺ form) to replace Na⁺ with K⁺. Collect the eluate and concentrate it under reduced pressure. Precipitate the ATP K⁺ salt by adding cold ethanol or acetone, then filter and wash with the same solvent. Dry the product under vacuum or lyophilize it. Confirm purity via HPLC or UV spectroscopy. Store at –20°C in a desiccator.

Usage Scenarios: ATP K+ salt (adenosine triphosphate potassium salt) is primarily used as an energy source in biochemical and physiological research. It serves as a substrate in enzymatic reactions, particularly those involving ATPases, kinases, and other ATP-dependent processes. In cell culture, it supports energy metabolism and maintains cellular functions. It is also utilized in muscle contraction studies, cardiac research, and mitochondrial function assays. Additionally, ATP K+ salt is employed in diagnostic kits for measuring ATP levels in clinical and laboratory settings. Its potassium counterion enhances solubility and stability, making it suitable for in vitro and ex vivo applications, including drug development and metabolic pathway analysis.

ATP K+ salt Basic Info

ATP K+ salt Price