bFGF amino acids 106-120

What is bFGF amino acids 106-120

bFGF amino acids 106-120 is a synthetic peptide fragment derived from the basic fibroblast growth factor (bFGF), a key protein involved in cell proliferation, tissue repair, and angiogenesis. This specific sequence, encompassing amino acids 106 to 120, plays a critical role in mediating the biological activities of bFGF, including its interaction with cell surface receptors and signaling pathways. Researchers and biotechnologists utilize this peptide fragment to study the molecular mechanisms of bFGF, develop targeted therapies, and explore its potential in regenerative medicine and wound healing. With its high purity and precise structure, bFGF amino acids 106-120 is an essential tool for advancing scientific understanding and therapeutic applications in biomedical research.

Preparation Process: To prepare the bFGF amino acid fragment 106-120, follow these steps: 1. **Solid-Phase Peptide Synthesis (SPPS)**: Use Fmoc chemistry on a resin (e.g., Rink amide MBHA). 2. **Coupling**: Activate Fmoc-protected amino acids (106-120 sequence: YRSRKYTSWYVALKR) with HBTU/DIPEA in DMF, coupling sequentially. 3. **Deprotection**: Remove Fmoc groups with 20% piperidine/DMF. 4. **Cleavage**: Treat the resin with TFA/TIS/water (95:2.5:2.5) for 2 hours to release the peptide. 5. **Purification**: Isolate the crude peptide via RP-HPLC (C18 column, acetonitrile/water gradient). 6. **Lyophilization**: Freeze-dry the purified peptide for storage. Verify purity by HPLC and mass spectrometry.

Usage Scenarios: The compound bFGF amino acids 106-120, a synthetic peptide fragment of basic fibroblast growth factor (bFGF), is primarily used in research to study cell proliferation, angiogenesis, and wound healing. It mimics a functional region of bFGF, allowing investigation of receptor binding and signaling mechanisms. This peptide is applied in vitro and in vivo to explore its role in tissue repair, neural regeneration, and cancer progression. It also aids in developing therapeutic strategies for conditions like ischemic injuries and degenerative diseases. Additionally, it serves as a tool for antibody production and competitive inhibition studies in biochemical assays.

bFGF amino acids 106-120 Basic Info

bFGF amino acids 106-120 Price