calf thymus DNA

What is calf thymus DNA

Calf thymus DNA is a high-purity, double-stranded DNA extracted from the thymus gland of calves, widely used in molecular biology and biochemical research. It serves as a versatile tool for studying DNA-protein interactions, enzyme kinetics, and nucleic acid structure. Due to its high molecular weight and stability, calf thymus DNA is often employed as a substrate for nucleases, polymerases, and other DNA-modifying enzymes. It is also utilized in assays to measure DNA-binding affinity, as a carrier in transfection experiments, and as a standard in quantitative DNA analysis. Its natural composition and reliable performance make it a valuable resource for advancing scientific understanding of genetic mechanisms.

Preparation Process: To prepare calf thymus DNA, first obtain fresh thymus tissue from a calf and mince it finely. Homogenize the tissue in a buffer (e.g., Tris-EDTA, pH 8.0) using a blender or homogenizer. Add protease (e.g., proteinase K) and SDS to digest proteins, then incubate at 55°C for 2–4 hours. Extract DNA by adding phenol-chloroform-isoamyl alcohol (25:24:1), centrifuge, and recover the aqueous phase. Precipitate DNA with cold ethanol or isopropanol, then spool the fibrous DNA onto a glass rod. Wash with 70% ethanol, air-dry, and dissolve in TE buffer. Assess purity via spectrophotometry (A260/A280 ratio ~1.8) and gel electrophoresis. Store at -20°C.

Usage Scenarios: Calf thymus DNA is primarily used as a standard reference in molecular biology experiments, such as spectrophotometric quantification of nucleic acids due to its high purity and known concentration. It serves as a substrate in enzymatic assays, including DNA polymerase, nuclease, and restriction enzyme activity tests. Researchers also use it in hybridization studies, gel electrophoresis calibration, and as a blocking agent in blotting techniques. Additionally, it aids in studying DNA-protein interactions, chromatin structure, and epigenetic modifications. Its availability in large quantities makes it valuable for educational purposes, allowing students to practice DNA extraction, purification, and analysis techniques.

calf thymus DNA Basic Info

calf thymus DNA Price